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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

Mouse Trib3 Antibody

CATALOG NUMBER: 57-631

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Mouse Trib3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the C-terminal region of mouse Trib3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Trib3
Additional Names:
Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, Trib3, Nipk, Trb3
Protein Accession Number:
Q8K4K2
Ncbi Gene Id Number:
228775
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Trib3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Background Reference 1:
Morse, E., et al. Am. J. Physiol. Renal Physiol. 299 (5), F965-F972 (2010) :
Background Reference 2:
Dedhia, P.H., et al. Blood 116(8):1321-1328(2010)
Background Reference 3:
Liew, C.W., et al. J. Clin. Invest. 120(8):2876-2888(2010)
Background Reference 4:
Humphrey, R.K., et al. J. Biol. Chem. 285(29):22426-22436(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-631

List Size:
400 ul

List Price:

$495.00

Datasheet
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