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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

Mouse Wee1 Antibody

CATALOG NUMBER: 60-265

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Wee1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of mouse Wee1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Wee1
Additional Names:
Wee1-like protein kinase, Wee1A kinase, Wee1
Protein Accession Number:
P47810
Ncbi Gene Id Number:
22390
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
Background Reference 1:
Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010) :
Background Reference 2:
Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008)
Background Reference 3:
Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007)
Background Reference 4:
Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-265

List Size:
400 ul

List Price:

$495.00

Datasheet
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