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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Mouse Wee2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

Mouse Wee2 Antibody

CATALOG NUMBER: 57-755

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Wee2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the Central region of mouse Wee2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Wee2
Additional Names:
Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase, mWee1B, Wee2, Wee1b
Protein Accession Number:
Q66JT0
Ncbi Gene Id Number:
381759
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphorylates and inhibits CDK1. May act as a negative regulator of entry into mitosis (G2 to M transition).
Background Reference 1:
Oh, J.S., et al. J. Cell Biol. 188(2):199-207(2010)
Background Reference 2:
Joshi, S., et al. BMC Dev. Biol. 7, 67 (2007) :
Background Reference 3:
Han, S.J., et al. Curr. Biol. 15(18):1670-1676(2005)
Background Reference 4:
Nakanishi, M., et al. Genes Cells 5(10):839-847(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-755

List Size:
400 ul

List Price:

$495.00

Datasheet
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