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<strong>Figure 1 WB Validation in Human Tonsil </strong><br>Loading: 15 μg of lysate. Antibodies: MOX1 5995, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mL
<strong>Figure 2 WB Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysate. Antibodies: MOX1 5995, 2 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: MOX1 5995, 2 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Figure 1 WB Validation in Human Tonsil
Loading: 15 μg of lysate. Antibodies: MOX1 5995, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mL
Trial Size

MOX1 Antibody

CATALOG NUMBER: 5995

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MOX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human MOX1.
The immunogen is located within amino acids 140 - 190 of MOX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 1-2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 28 kD Observed: 28 kD
Purification:
MOX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MOX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MOX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MEOX1
Additional Names:
MOX1 Antibody: KFS2, MOX1, Homeobox protein MOX-1, Mesenchyme homeobox 1
Protein Accession Number:
NP_004518
Protein Gi Number:
4758710
Ncbi Gene Id Number:
4222
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MOX1 Antibody: MOX1, also known as MEOX1, is a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes that is first expressed during gastrulation in the early mesoderm and later in presomitic mesoderm, early somites, dermomyotome and sclerotome. Both MOX1 and the related protein MOX2 are required for proper gene expression in all somitic compartments. MOX1 is an upstream regulator of the transcription factors Bapx1, Tbx18, and Uncx and is thus part of a regulatory circuit that functions in the maintenance of rostrocaudal sclerotome polarity and axial skeleton formation.
Background Reference 1:
Cadia AF, Hu J, Crosby J, et al. Mox-1 and Mox-2 define a novel homeobox gene subfamily and are differentially expressed during early mesodermal patterning in mouse embryos. Development1992; 116:1123-36.
Background Reference 2:
Candia AF and Wright CV. Differential localization of Mox-1 and Mox-2 proteins indicates distinct roles during development. Int. J. Dev. Biol.1996; 40:1179-84.
Background Reference 3:
Mankoo BS, Skuntz S, Harrigan I, et al. The concerted action of Meox homeobox genes is required upstream of genetic pathways essential for the formation, patterning, and differentiation of somites. Development2003; 130:4655-64.
Background Reference 4:
Skuntz S, Mankoo B, Nguyen MT, et al. Lack of the mesodermal homeodomain protein MEOX1 disrupts sclerotome polarity and leads to a remodeling of the craniocervical joints of the axial skeleton. Dev. Biol.2009; 332:383-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5995

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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