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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis of MPP1 using rabbit polyclonal MPP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.
MPP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

MPP1 Antibody

CATALOG NUMBER: 55-360

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-327 amino acids from the Central region of human MPP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MPP1
Additional Names:
55 kDa erythrocyte membrane protein, p55, Membrane protein, palmitoylated 1, MPP1, DXS552E, EMP55
Protein Accession Number:
Q00013
Protein Gi Number:
1346575
Ncbi Gene Id Number:
4354
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the prototype of the membrane-associatedguanylate kinase (MAGUK) family proteins. MAGUKs interact with thecytoskeleton and regulate cell proliferation, signaling pathways,and intercellular junctions. The encoded protein is an extensivelypalmitoylated membrane phosphoprotein containing a PDZ domain, aSrc homology 3 (SH3) motif, and a guanylate kinase domain. Thisgene product interacts with various cytoskeletal proteins and celljunctional proteins in different tissue and cell types, and may beinvolved in the regulation of cell shape, hair cell development,neural patterning of the retina, and apico-basal polarity and tumorsuppression pathways in non-erythroid cells. Multiple transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Seo, P.S., et al. Exp. Biol. Med. (Maywood) 234(3):255-262(2009)
Background Reference 2:
Seo, P.S., et al. Biochim. Biophys. Acta 1793(2):281-289(2009)
Background Reference 3:
Gosens, I., et al. Hum. Mol. Genet. 16(16):1993-2003(2007)
Background Reference 4:
Kusunoki, H., et al. Proteins 64(3):804-807(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-360

List Size:
400 ul

List Price:

$495.00

Datasheet
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