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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

MPP3 Antibody

CATALOG NUMBER: 56-830

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MPP3
Additional Names:
MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3, MPP3, DLG3
Protein Accession Number:
Q13368
Protein Gi Number:
150421601
Ncbi Gene Id Number:
4356
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product is a member of a family ofmembrane-associated proteins termed MAGUKs (membrane-associatedguanylate kinase homologs). MAGUKs interact with the cytoskeletonand regulate cell proliferation, signaling pathways, andintracellular junctions. This protein contains a conservedsequence, called the SH3 (src homology 3) motif, found in severalother proteins that associate with the cytoskeleton and aresuspected to play important roles in signal transduction.Alternatively spliced transcript variants have been identified. Onetranscript variant is experimentally supported, but it doesn'tencode a protein.
Background Reference 1:
Qin, L., et al. Zhonghua Gan Zang Bing Za Zhi 15(7):509-512(2007)
Background Reference 2:
Kantardzhieva, A., et al. FEBS J. 273(6):1152-1165(2006)
Background Reference 3:
Stelzl, U., et al. Cell 122(6):957-968(2005)
Background Reference 4:
Fukuhara, H., et al. Oncogene 22(40):6160-6165(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-830

List Size:
400 ul

List Price:

$495.00

Datasheet
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