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42-674 (2ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-674 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-674 (2ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MPV17 Antibody

CATALOG NUMBER: 42-674

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QRALAAHPWKVQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 19kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 19.7kDa according to NP_002428.1). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.. Recommended concentration, 5-10ug/ml.
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MPV17
Additional Names:
MPV17, MpV17 mitochondrial inner membrane protein, SYM1, Mpv17 protein, Mpv17, human homolog of glomerulosclerosis and nephrotic syndrome, OTTHUMP00000122591
Protein Accession Number:
NP_002428.1
Protein Gi Number:
4505241
Ncbi Gene Id Number:
4358

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-674

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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