Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: MPYS 5269 (1 μg/mL), MPYS 64-236 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: MPYS 5269 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human THP-1 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse A-20 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: MPYS 5269 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunocytochemistry Validation of MPYS in Mouse A20 Cells</strong><br>Immunocytochemical analysis of A20 cells using anti-MPYS antibody (5269) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of MPYS in Moue A20 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with 5269 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: MPYS 5269 (1 μg/mL), MPYS 64-236 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

MPYS Antibody

CATALOG NUMBER: 5269

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat (82%), Bovine: (82%), Pig: (82%), Chicken: (71%)
Immunogen:
Anti-MPYS antibody (5269) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS.
The immunogen is located within amino acids 260-310 of MPYS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 4:
Cat. No. 17-208 - A-20 Cell Slide
Predicted Molecular Weight:
Predicted: 42kD
Observed: 42 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar MPYS expression profile in human cell lines detected by two independent anti-MPYS antibodies that recognize different epitopes, 5269 against near C-terminus domain and 64-236 against C-terminus domain.  MPYS proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human MPYS has one isoform (379aa, 42kD). Mouse MPYS has 3 isoforms, including isoform 1 (379aa, 42kD), isoform 2 (427aa, 48kD) and isoform 3 (337aa, 38kD). Rat MPYS also has one isoform (379aa, 43kD). 5269 can detect human, mouse and rat.
Purification:
MPYS Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MPYS antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMEM173
Additional Names:
MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING
Protein Accession Number:
NP_938023
Protein Gi Number:
38093659
Ncbi Gene Id Number:
340061
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Background Reference 1:
Jin et al. Mol. Cell. Biol.2008; 28:5014-26.
Background Reference 2:
Zhong et al. Immunity2008; 29:538-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5269

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369