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Western blot analysis of extracts of various cell lines, using MRE11A antibody (18-710) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug MRE11A antibody (18-710). Western blot was performed from the immunoprecipitate using MRE11A antibody (18-710) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using MRE11A antibody (18-710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MRE11A Antibody

CATALOG NUMBER: 18-710

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MRE11A (NP_005581.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
Raji
Positive Control 2:
SW480
Positive Control 3:
U-937
Positive Control 4:
A-549
Positive Control 5:
A-375
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 90kDa-100kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MRE11A
Additional Names:
MRE11 Antibody: ATLD, HNGS1, MRE11, MRE11B, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1
Ncbi Gene Id Number:
4361
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-710

List Size:
100 uL

List Price:

$436.00

Datasheet
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