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45-901 (0.3ug/ml) staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-901 (0.3ug/ml) staining of Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-901 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-901 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-901 (0.3ug/ml) staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

MRG15 Antibody

CATALOG NUMBER: 45-901

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: APKQDPKPKFQEG-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in nuclear lysates of cell lines HepG2 and NIH3T3 and approx 40kDa band observed in Mouse, Rat and Pig Liver lysates (calculated MW of 37.2kDa according to Human NP_006782.1, Mouse NP_077751.1, Rat NP_001011999.1 and Pig XP_001927776.1 and 41.5kDa according to Human NP_996670.1, Mouse NP_001034236.1 and Pig XP_001927789.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MORF4L1
Additional Names:
MORF4L1, MRG15, MORF-related gene 15, mortality factor 4 like 1, Eaf3, FWP006, HsT17725, MEAF3, MGC10631, MORFRG15, Esa1p-associated factor 3 homolog, MORF-related gene on chromosome 15
Protein Accession Number:
NP_006782.1,NP_996670.1
Protein Gi Number:
5803102
Ncbi Gene Id Number:
10933
Background Reference 1:
Pardo PS, Leung JK, Lucchesi JC, Pereira-Smith OM. MRG15, a Novel Chromodomain Protein, Is Present in Two Distinct Multiprotein Complexes Involved in Transcriptional Activation. J Biol Chem. 2002 Dec 27;277(52):50860-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-901

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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