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45-903 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-903 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results with antigen retrieval at pH9.
45-903 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-903 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MRP5 Antibody

CATALOG NUMBER: 45-903

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: KDIDIGKEYIIP-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 161kDa according to NP_005679.2). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in DCT. Paraffin embedded Human Liver. Recommended concentration: 4-6ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005679.2; NP_001018881.1).
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCC5
Additional Names:
MRP5, DKFZp686C1782, ABCC5, ATP-binding cassette, sub-family C (CFTR/MRP), member 5, ABC33, EST277145, MOAT-C, MOATC, SMRP, pABC11, ATP-binding cassette, sub-family C, member 5, canalicular multispecific organic anion transporter C
Protein Accession Number:
NP_005679.2,NP_001018881.1
Protein Gi Number:
66529005
Ncbi Gene Id Number:
10057
Background Reference 1:
Meyer Zu Schwabedissen HE, Grube M, Heydrich B, Linnemann K, Fusch C, Kroemer HK, Jedlitschky G. Expression, localization, and function of MRP5 (ABCC5), a transporter for cyclic nucleotides, in human placenta and cultured human trophoblasts: effects of gestational age and cellular differentiation Am J Pathol. 2005 Jan;166(1):39-48.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-903

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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