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46-868 (0.5ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-868 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-868 (0.5ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MRP8 Antibody

CATALOG NUMBER: 46-868

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KVVEFDRPEVLRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 125kDa band observed in Human Breast and Human Testis lysates (calculated MW of 150kDa according to NP_660187.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_115972.2; NP_660187.1). Reported variants represent identical protein (NP_115972.2; NP_149163.2).
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABCC11
Additional Names:
ABCC11, ATP-binding cassette transporter MRP8, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8, WW, ATP-binding cassette protein C11, ATP-binding cassette, sub-family C, member 11, multi-resistance protein 8
Protein Accession Number:
NP_115972.2,NP_660187.1
Protein Gi Number:
21729873
Ncbi Gene Id Number:
85320
Background Reference 1:
Bortfeld M, Rius M, König J, Herold-Mende C, Nies AT, Keppler D. Human multidrug resistance protein 8 (MRP8/ABCC11), an apical efflux pump for steroid sulfates, is an axonal protein of the CNS and peripheral nervous system. Neuroscience. 2006;137(4):1247-57

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-868

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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