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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

MRPL54 Antibody

CATALOG NUMBER: 59-389

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MRPL54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the Central region of human MRPL54.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MRPL54
Additional Names:
39S ribosomal protein L54, mitochondrial, L54mt, MRP-L54, MRPL54
Protein Accession Number:
Q6P161
Protein Gi Number:
74737109
Ncbi Gene Id Number:
116541
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mammalian mitochondrial ribosomal proteins are encoded bynuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of asmall 28S subunit and a large 39S subunit. They have an estimated75% protein to rRNA composition compared to prokaryotic ribosomes,where this ratio is reversed. Another difference between mammalianmitoribosomes and prokaryotic ribosomes is that the latter containa 5S rRNA. Among different species, the proteins comprising themitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequencehomology. This gene encodes a 39S subunit protein. [provided byRefSeq].
Background Reference 1:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 2:
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Background Reference 3:
Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
Background Reference 4:
Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-389

List Size:
400 ul

List Price:

$495.00

Datasheet
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