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Western blot analysis in WiDr cell line lysates (35ug/lane).
MRPL9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in WiDr cell line lysates (35ug/lane).

MRPL9 Antibody

CATALOG NUMBER: 56-813

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MRPL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human MRPL9.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MRPL9
Additional Names:
39S ribosomal protein L9, mitochondrial, L9mt, MRP-L9, MRPL9
Protein Accession Number:
Q9BYD2
Protein Gi Number:
209572671
Ncbi Gene Id Number:
65005
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mammalian mitochondrial ribosomal proteins are encoded bynuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of asmall 28S subunit and a large 39S subunit. They have an estimated75% protein to rRNA composition compared to prokaryotic ribosomes,where this ratio is reversed. Another difference between mammalianmitoribosomes and prokaryotic ribosomes is that the latter containa 5S rRNA. Among different species, the proteins comprising themitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequencehomology. This gene encodes a 39S subunit protein. A pseudogenecorresponding to this gene is found at 8q21.11. [provided byRefSeq].
Background Reference 1:
Naukkarinen, J., et al. PLoS Genet.(6), E1000976 (2010) :
Background Reference 2:
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Background Reference 3:
Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
Background Reference 4:
Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-813

List Size:
400 ul

List Price:

$495.00

Datasheet
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