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45-092 (0.05ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-092 (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-092 (0.05ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MS4A1 Antibody

CATALOG NUMBER: 45-092

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CQDQESSPIENDSSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Lymph Node lysates (calculated MW of 33.1kDa according to NP_068769.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Tonsil shows membranous staining mainly in the Germinal Center. Recommended concentration: 2-4ug/ml.
Specificity:
Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MS4A1
Additional Names:
MS4A1, membrane-spanning 4-domains, subfamily A, member 1, B1, S7, BP35, CD20, LEU-16, MGC3969, CD20 antigen, CD20 receptor, B-lymphocyte cell-surface antigen B1
Protein Accession Number:
NP_068769.2,NP_690605.1
Protein Gi Number:
23110987
Ncbi Gene Id Number:
931
Background Reference 1:
Stamenkovic I, Seed B. Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein. J Exp Med 1988 Jun 1;167(6):1975-80

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-092

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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