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Western Blot at 1:1000 dilution + Ramos whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse heart tissue lysates (35ug/lane).
Western Blot at 1:1000 dilution + Ramos whole cell lysates Lysates/proteins at 20 ug per lane.

MSC Antibody

CATALOG NUMBER: 59-515

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the C-terminal region of human MSC.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSC
Additional Names:
Musculin, Activated B-cell factor 1, ABF-1, Class A basic helix-loop-helix protein 22, bHLHa22, MSC, ABF1, BHLHA22
Protein Accession Number:
O60682
Ncbi Gene Id Number:
9242
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transcriptionalrepressor capable of binding an E-box element either as a homodimeror as a heterodimer with E2A in vitro. The encoded protein alsoforms heterodimers with E2A proteins in vivo. This protein iscapable of inhibiting the transactivation capability of E47, an E2Aprotein, in mammalian cells. This gene is a downstream target ofthe B-cell receptor signal transduction pathway. [provided byRefSeq].
Background Reference 1:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 2:
Ushmorov, A., et al. Leukemia 22(10):1942-1944(2008)
Background Reference 3:
Knight, J.C., et al. Nat. Genet. 36(4):394-399(2004)
Background Reference 4:
Wong, J., et al. DNA Cell Biol. 20(8):465-471(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-515

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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