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Western blot analysis of extracts of various cell lines, using MSH2 antibody (13-955) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (13-955). Western blot was performed from the immunoprecipitate using MSH2 antibody (13-955) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using MSH2 antibody (13-955) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

MSH2 Antibody

CATALOG NUMBER: 13-955

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
NIH/3T3
Positive Control 2:
A431
Positive Control 3:
A549
Positive Control 4:
Mouse testis
Positive Control 5:
Mouse thymus
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MSH2
Additional Names:
MSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1
Ncbi Gene Id Number:
4436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-955

List Size:
100 uL

List Price:

$436.00

Datasheet
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