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Western blot analysis using MSH2 monoclonal antibody against A431 cell lysate.
Immunohistochemical analysis of paraffin - embedded human rectum carcinoma tissue showing nuclear and cytoplasmic localization using MSH2 antibody with DAB staining.
Western blot analysis using MSH2 monoclonal antibody against A431 cell lysate.

MSH2 Antibody [3A2B8C]

CATALOG NUMBER: 32-196

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
MSH2 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSH2
Additional Names:
MutS protein homolog 2, hMSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1
Protein Accession Number:
P43246
Protein Gi Number:
1171032
Ncbi Gene Id Number:
4436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Background Reference 1:
Papadopoulos, N. 1994. Science 263: 1625-1629.
Background Reference 2:
Palombo, F. 1994. Nature 367:417-418.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-196

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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