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Western blot analysis in SW480,U251 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of MSH2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in SW480,U251 cell line lysates (35ug/lane).

MSH2 Antibody

CATALOG NUMBER: 55-920

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the Central region of human MSH2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSH2
Additional Names:
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Protein Accession Number:
P43246
Protein Gi Number:
1171032
Ncbi Gene Id Number:
4436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MSH2 was identified as a locus frequently mutated inhereditary nonpolyposis colon cancer (HNPCC). When cloned, it wasdiscovered to be a human homolog of the E. coli mismatch repairgene mutS, consistent with the characteristic alterations inmicrosatellite sequences (RER+ phenotype) found in HNPCC. [providedby RefSeq].
Background Reference 1:
Kim, M., et al. Cancer Sci. 101(11):2436-2442(2010)
Background Reference 2:
Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010) In press :
Background Reference 3:
Srivastava, K., et al. Cancer 116(13):3160-3169(2010)
Background Reference 4:
van der Post, R.S., et al. J. Med. Genet. 47(7):464-470(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-920

List Size:
400 ul

List Price:

$495.00

Datasheet
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