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Western blot analysis of lysate from SH-SY5Y cell line, using MSI1 Antibody at 1:1000 at each lane.
MSI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from SH-SY5Y cell line, using MSI1 Antibody at 1:1000 at each lane.

MSI1 Antibody

CATALOG NUMBER: 56-147

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MSI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the N-terminal region of human MSI1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSI1
Additional Names:
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Protein Accession Number:
O43347
Ncbi Gene Id Number:
4440
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein containing two conservedtandem RNA recognition motifs. Similar proteins in other speciesfunction as RNA-binding proteins and play central roles inposttranscriptional gene regulation. Expression of this gene hasbeen correlated with the grade of the malignancy and proliferativeactivity in gliomas and melanomas. A pseudogene for this gene islocated on chromosome 11q13.
Background Reference 1:
Fan, L.F., et al. IntColorectal Dis 25(1):17-23(2010)
Background Reference 2:
de Sousa Abreu, R., et al. J. Biol. Chem. 284(18):12125-12135(2009)
Background Reference 3:
Murayama, M., et al. J. Gastroenterol. 44(3):173-182(2009)
Background Reference 4:
Gotte, M., et al. J. Pathol. 215(3):317-329(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-147

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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