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43-609 (2ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-609 (1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-609 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-609 (2ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MSI2 / musashi-2 Antibody

CATALOG NUMBER: 43-609

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: QGTSGSANDSQHD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Approx 35+37kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to NP_473384.1 ). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform a (NP_620412.1) only.
Predicted Molecular Weight:
Approx 37kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MSI2 / musashi-2
Additional Names:
MSI2, musashi RNA-binding protein 2, MSI2H, RNA-binding protein Musashi homolog 2, musashi homolog 2, musashi-2
Protein Accession Number:
NP_620412.1
Protein Gi Number:
20373175
Ncbi Gene Id Number:
124540
Background Reference 1:
Sakakibara S, Nakamura Y, Satoh H, Okano H.Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-609

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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