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Western blot analysis of extracts from NIH-3T3 cells treated with EGF using MSK1 (Phospho-Ser212) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from NIH-3T3 cells treated with EGF using MSK1 (Phospho-Ser212) Antibody. The lane on the right is treated with the antigen-specific peptide.

MSK1 (phospho Ser212) Antibody

CATALOG NUMBER: 79-984

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
MSK1 (phospho Ser212) antibody was raised against a peptide sequence around phosphorylation site of serine 212 (A-Y-S (p)-F-C) derived from Human MSK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of MSK1 only when phosphorylated at serine 212.
Predicted Molecular Weight:
90 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RPS6KA5
Additional Names:
polypeptide 5, KS6A5, MSPK1, RPS6KA5, RLPK
Protein Accession Number:
NP_004746.2
Protein Gi Number:
32528295
Ncbi Gene Id Number:
9252
Background:
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
Background Reference 1:
McCoy CE, et al. (2007) Biochem J 402, 491-501
Background Reference 2:
McCoy CE, et al. (2005)Biochem J 387, 507-17

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-984

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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