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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

MSLN Antibody

CATALOG NUMBER: 25-753

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSLN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MSLN antibody can be used for detection of MSLN by ELISA at 1:312500. MSLN antibody can be used for detection of MSLN by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
32 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MSLN antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MSLN
Additional Names:
MSLN, CAK1, MPF, SMR, SMRP
Protein Accession Number:
NP_005814
Protein Gi Number:
53988378
Ncbi Gene Id Number:
10232
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin.An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.
Background Reference 1:
Johnson, M.D., (2008) J. Histochem. Cytochem. 56 (6), 579-585.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-753

List Size:
100 ul

List Price:

$519.00

Datasheet
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