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45-909 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-909 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-909 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

MSX1 Antibody

CATALOG NUMBER: 45-909

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: TSLPLGVKVEDS-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4ug/ml. Data obtained from a previous batch (different goat).
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Prostate Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MSX1
Additional Names:
MSX1, HOX7, HYD1, msh homeo box homolog 1 (Drosophila), msh homeobox 1, STHAG1, homeobox 7, msh homeo box 1, msh homeobox homolog 1
Protein Accession Number:
NP_002439.2
Protein Gi Number:
118582284
Ncbi Gene Id Number:
4487
Background Reference 1:
Blin-Wakkach C, Lezot F, Ghoul-Mazgar S, Hotton D, Monteiro S, Teillaud C, Pibouin L, Orestes-Cardoso S, Papagerakis P, Macdougall M, Robert B, Berdal A. Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals. Proc Natl Acad Sci USA. 2001 Jun 19;98(13):7336-41. Epub 2001 Jun 05.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-909

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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