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Western Blot at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane.
Fluorescent image of U251 cell stained with MSX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MSX1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in A375 cell line lysates (35ug/lane).
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Fluorescent confocal image of SY5Y cells stained with MSX1 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MSX1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.
Western Blot at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane.

MSX1 Antibody

CATALOG NUMBER: 60-419

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:2000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MSX1
Additional Names:
Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
Protein Accession Number:
P28360
Protein Gi Number:
557952603
Ncbi Gene Id Number:
4487
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the muscle segment homeoboxgene family. The encoded protein functions as a transcriptionalrepressor during embryogenesis through interactions with componentsof the core transcription complex and other homeoproteins. It mayalso have roles in limb-pattern formation, craniofacialdevelopment, particularly odontogenesis, and tumor growthinhibition. Mutations in this gene, which was once known ashomeobox 7, have been associated with nonsyndromic cleft lip withor without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome,and autosomoal dominant hypodontia.
Background Reference 1:
References for protein:
Background Reference 2:
Sliwinski, T., et al. Cancer Epidemiol 34(5):652-655(2010)
Background Reference 3:
Nikopensius, T., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(9):748-756(2010)
Background Reference 4:
Suazo, J., et al. Am. J. Med. Genet.152A (8), 2011-2016 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-419

List Size:
400 ul

List Price:

$495.00

Datasheet
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