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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

MTA1 Antibody

CATALOG NUMBER: 60-671

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This MTA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 659-687 amino acids from the C-terminal region of human MTA1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
81 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MTA1
Additional Names:
Metastasis-associated protein MTA1, MTA1
Protein Accession Number:
Q13330
Protein Gi Number:
259016275
Ncbi Gene Id Number:
9112
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that was identified in ascreen for genes expressed in metastatic cells, specifically,mammary adenocarcinoma cell lines. Expression of this gene has beencorrelated with the metastatic potential of at least two types ofcarcinomas although it is also expressed in many normal tissues.The role it plays in metastasis is unclear. It was initiallythought to be the 70kD component of a nucleosome remodelingdeacetylase complex, NuRD, but it is more likely that thiscomponent is a different but very similar protein. These twoproteins are so closely related, though, that they share the sametypes of domains. These domains include two DNA binding domains, adimerization domain, and a domain commonly found in proteins thatmethylate DNA. The profile and activity of this gene productsuggest that it is involved in regulating transcription and thatthis may be accomplished by chromatin remodeling. [provided byRefSeq].
Background Reference 1:
Zhu, X., et al.Thorac Oncol 5(8):1159-1166(2010)
Background Reference 2:
Van Rechem, C., et al. Mol. Cell. Biol. 30(16):4045-4059(2010)
Background Reference 3:
Yang, Y.M., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):682-684(2010)
Background Reference 4:
Li, D.Q., et al. J. Biol. Chem. 285(26):19802-19812(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-671

List Size:
400 ul

List Price:

$495.00

Datasheet
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