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Western blot analysis of extracts of various cell lines, using MTAP antibody (13-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using MTAP antibody (13-684). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MTAP antibody (13-684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MTAP Antibody

CATALOG NUMBER: 13-684

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
22Rv1
Positive Control 2:
293T
Positive Control 3:
Mouse liver
Predicted Molecular Weight:
Observed: 31kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MTAP
Additional Names:
BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MeSAdo phosphorylase, epididymis luminal protein 249, epididymis secretory sperm binding protein
Ncbi Gene Id Number:
4507
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-684

List Size:
100 uL

List Price:

$436.00

Datasheet
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