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Western blot analysis of MTERFD2 in human small intestine tissue lysate with MTERFD2 antibody at 1 μg/mL.
Immunohistochemistry of MTERFD2 in human small intestine tissue with MTERFD2 antibody at 2.5 μg/ml.
Immunofluorescence of MTERFD2 in human small intestine tissue with MTERFD2 antibody at 20 μg/ml.
Western blot analysis of MTERFD2 in human small intestine tissue lysate with MTERFD2 antibody at 1 μg/mL.
Trial Size

MTERFD2 Antibody

CATALOG NUMBER: 7283

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MTERFD2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD2 .
The immunogen is located within amino acids 260 - 310 of MTERFD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MTERFD2 Antibody can be used for detection of MTERFD2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of MTERFD2 are known to exist. MTERFD2 antibody is predicted to not cross-react with other MTERFD protein family members.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 42 kDa
Observed: 44 kDa
Purification:
MTERFD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MTERFD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MTERFD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MTERFD2
Additional Names:
MTERFD2 Antibody: MTERF4, mTERF domain-containing protein 2, Mitochondrial transcription termination factor 4, MTERF4
Protein Accession Number:
NP_872307
Protein Gi Number:
42740903
Ncbi Gene Id Number:
130916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MTERFD2 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD2 is targeted to the mitochondria and is ubiquitously expressed, with highest expression levels in fore- and midbrain, diencephalon, spinal cord, tongue, lung liver and kidney. MTERFD2 has been suggested to play a role in organ differentiation during embryogenesis. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
Background Reference 1:
Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
Background Reference 2:
Xu Q, Zhang F, He H, et al. Expression profile of mouse Mterfd2, a novel component of the mitochondrial transcription termination (MTERF) family. Genes Genet. Syst. 2011; 86:269-75.
Background Reference 3:
Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7283

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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