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Western blot analysis of MTERFD3 in human testis tissue lysate with MTERFD3 antibody at 1 μg/mL.
Immunohistochemistry of MTERFD3 in mouse testis tissue with MTERFD3 antibody at 2.5 μg/ml.
Western blot analysis of MTERFD3 in human testis tissue lysate with MTERFD3 antibody at 1 μg/mL.
Trial Size

MTERFD3 Antibody

CATALOG NUMBER: 7285

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (76%)
Immunogen:
MTERFD3 antibody was raised against an 18 amino acid peptide near the amino terminus of human MTERFD3 .
The immunogen is located within amino acids 20 - 70 of MTERFD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
MTERFD3 Antibody can be used for detection of MTERFD3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
MTERFD3 antibody is predicted to not cross-react with other MTERFD protein family members.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 42 kDa
Observed: 40 kDa
Purification:
MTERFD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MTERFD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MTERFD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MTERFD3
Additional Names:
MTERFD3 Antibody: mTERF2, mTERFL, mTERF domain-containing protein 3, mitochondrial, Mitochondrial transcription termination factor 2, mTERF2
Protein Accession Number:
NP_079474
Protein Gi Number:
21314736
Ncbi Gene Id Number:
80298
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MTERFD3 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD3 was initially identified through differential display analysis and is expressed primarily in heart, liver, pancreas and skeletal muscle. MTERFD3 is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription, and may be a serum-inhibitory factor.
Background Reference 1:
Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
Background Reference 2:
Chen Y, Zhou G, Yu M, et al. Cloning and functional analysis of human mTERFL encoding a novel mitochondrial transcription termination factor-like protein. Biochem. Biophys. Res. Commun. 2005; 337:1112-8.
Background Reference 3:
Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7285

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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