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Antibody used in WB on Human HepG2 at 5.0 ug/ml.
Antibody used in WB on Human HepG2 at 5.0 ug/ml.

MTF1 Antibody

CATALOG NUMBER: 27-481

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MTF1 antibody can be used for detection of MTF1 by ELISA at 1:2500. MTF1 antibody can be used for detection of MTF1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
81 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MTF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MTF1
Additional Names:
MTF1, ZRF, MTF-1
Protein Accession Number:
NP_005946
Protein Gi Number:
112790163
Ncbi Gene Id Number:
4520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The zinc finger transcription factor MTF-1 (metal-responsive transcription factor-1) is conserved from insects to vertebrates. The major role of MTF-1 in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu2+, Zn2+ and Cd2+. In mammals, MTF-1 serves at least two additional roles. First, targeted disruption of the MTF-1 gene results in death at embryonic day 14 due to liver degeneration, revealing a stage-specific developmental role. Second, under hypoxic-anoxic stress, MTF-1 helps to activate the transcription of the gene placental growth factor (PIGF), an angiogenic protein.
Background Reference 1:
Chen, X., et al., (2004) J. Biol. Chem. 279 (6), 4515-4522.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-481

List Size:
100 ul

List Price:

$473.00

Datasheet
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