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Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

MTHFD2 Antibody

CATALOG NUMBER: 13-620

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
U-87MG
Positive Control 3:
HT-29
Positive Control 4:
293T
Positive Control 5:
DU145
Positive Control 6:
Mouse pancreas
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MTHFD2
Additional Names:
MTHFD2, NMDMC
Ncbi Gene Id Number:
10797
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-620

List Size:
100 uL

List Price:

$436.00

Datasheet
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