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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

MTHFD2 Antibody

CATALOG NUMBER: 26-095

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTHFD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MTHFD2 antibody can be used for detection of MTHFD2 by ELISA at 1:312500. MTHFD2 antibody can be used for detection of MTHFD2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
35 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MTHFD2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MTHFD2
Additional Names:
MTHFD2, NMDMC
Protein Accession Number:
NP_006627
Protein Gi Number:
94721354
Ncbi Gene Id Number:
10797
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This protein is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.
Background Reference 1:
Hillier, L.W., (2005) Nature 434 (7034), 724-731.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-095

List Size:
100 ul

List Price:

$519.00

Datasheet
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