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45-910 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-910 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-910 (1ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-910 (10ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-910 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MTM1 Antibody

CATALOG NUMBER: 45-910

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SSPSQMMPHVQTHF
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in lysates of Human and Mouse Skeletal Muscle and of cell line HepG2 (calculated MW of 69.9kDa according to NP_000243.1and 69.6kDa according to Mouse NP_001157662.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MTM1
Additional Names:
MTM1, myotubular myopathy 1, CNM, MTMX, XLMTM, myotubularin, myotubularin 1
Protein Accession Number:
NP_000243.1
Protein Gi Number:
4557896
Ncbi Gene Id Number:
4534
Background Reference 1:
Laporte J, Hu LJ, Kretz C, Mandel JL, Kioschis P, Coy JF, Klauck SM, Poustka A, Dahl N. A gene mutated in X-linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast. Nat Genet. 1996 Jun;13(2):175-82.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-910

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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