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46-987 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-987 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MTNR1A Antibody

CATALOG NUMBER: 46-987

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SLKYDKLYSSKNS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in Rat Retina lysates (calculated MW of 39.8kDa according to Rat NP_446128.1). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control 1:
KELLY (Human neuroblastoma cell line) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MTNR1A
Additional Names:
MTNR1A, melatonin receptor 1A, MEL-1A-R, MT1, melatonin receptor type 1A
Protein Accession Number:
NP_005949.1
Protein Gi Number:
5174593
Ncbi Gene Id Number:
4543
Background Reference 1:
Rawashdeh O, de Borsetti NH, Roman G, Cahill GM. Melatonin suppresses nighttime memory formation in zebrafish. Science 2007 Nov 318 (5853): 1144-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-987

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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