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Western blot analysis of MUC20 using rabbit polyclonal MUC20 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MUC20 gene (Lane 2).
Confocal immunofluorescent analysis of MUC20 Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of MUC20 using rabbit polyclonal MUC20 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MUC20 gene (Lane 2).

MUC20 Antibody

CATALOG NUMBER: 63-223

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human MUC20.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MUC20
Additional Names:
Mucin-20, MUC-20, MUC20, KIAA1359
Protein Accession Number:
Q8N307
Protein Gi Number:
317373415
Ncbi Gene Id Number:
200958
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in different individuals. Multiple transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Li,G., Am. J. Nephrol. 26 (1), 43-49 (2006)
Background Reference 2:
Suzuki,Y., Genome Res. 14 (9), 1711-1718 (2004)
Background Reference 3:
Higuchi,T., Mol. Cell. Biol. 24 (17), 7456-7468 (2004)
Background Reference 4:
Higuchi,T., J. Biol. Chem. 279 (3), 1968-1979 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-223

List Size:
400 ul

List Price:

$495.00

Datasheet
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