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IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).
IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).

Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]

CATALOG NUMBER: 33-241

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Frog, Goat, Guinea Pig, Human, Marmoset, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Frog, Goat, Guinea Pig, Human, Marmoset, Mouse, Pig, Rat
Immunogen:
A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Gene Id Number:
3851(CK4);3852(CK5);3853(CK6);3856(CK8);3858(CK10);3860(CK13);3875(CK18)
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-241

List Size:
100 ug

List Price:

$626.00

Datasheet
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