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Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Munc-18 (phospho Ser515) Antibody

CATALOG NUMBER: 50-230

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Dog, Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Dog, Frog, Human, Mouse, Rat
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser515 of Munc-18.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Predicted Molecular Weight:
65
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
Munc-18 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Stxbp2
Protein Accession Number:
Q62753
Protein Gi Number:
2501516
Ncbi Gene Id Number:
81804
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Background Reference 1:
Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
Background Reference 2:
Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
Background Reference 3:
Sassa T, Ogawa H, Kimoto M, Hosono R (1996) The synaptic protein UNC-18 is phosphorylated by protein kinase C. Neurochem Int 29:543-552.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-230

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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