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45-916 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-916 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-916 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MUNC18 Antibody

CATALOG NUMBER: 45-916

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-SRVSFEDQAPTME
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human and Mouse Brain lysates (calculated MW of 68.7kDa according to NP_003156.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain cortex shows vesicular staining in neuronal bodies. Recommended concentration: 5-10ug/ml. This antibody has been successfully used in IHC: Meijer M et al, PLoS One. 2015 Sep 25;10(9):e0138950. PMID: 26407320.
Specificity:
This antibody is expected to recognize isoform a (NP_003156.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STXBP1
Additional Names:
STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding protein 1, Hunc18, rbSec1, syntaxin-binding protein 1, RP11-56D16.3, EIEE4, FLJ37475, RBSEC1
Protein Accession Number:
NP_003156.1
Protein Gi Number:
4507297
Ncbi Gene Id Number:
6812
Background Reference 1:
Swanson DA, Steel JM, Valle D. Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release. Genomics. 1998 Mar 15;48(3):373-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-916

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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