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Western blot analysis of anti-MUSK Pab in mouse kidney tissue lysates (35ug/lane)
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of MUSK Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-MUSK Pab in mouse kidney tissue lysates (35ug/lane)

MUSK Antibody

CATALOG NUMBER: 63-115

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MUSK antibody is generated from rabbits immunized with human MUSK recombinant protein.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MUSK
Additional Names:
Muscle, skeletal receptor tyrosine-protein kinase, Muscle-specific tyrosine-protein kinase receptor, MuSK, Muscle-specific kinase receptor, MUSK
Protein Accession Number:
O15146
Ncbi Gene Id Number:
4593
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MuSK activates signaling cascades responsible for many aspects of synapse formation, including the organization of the postsynaptic membrane, synapse-specific transcription, and presynaptic differentiation. MuSK also mediates agrin-induced aggregation of acetylcholine receptors at the mature vertebrate neuromuscular junction and also during synapse formation. It has been shown that a majority of AChR Ab-seronegative myasthenia gravis patients possess serum autoantibodies against MuSK.
Background Reference 1:
Blume-Jensen P, et al. Nature 2001. 411: 355.
Background Reference 2:
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Background Reference 3:
JhiangOncogene 2000. 19: 5590.
Background Reference 4:
Manning G, et al. Science 2002. 298: 1912.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-115

List Size:
400 ul

List Price:

$495.00

Datasheet
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