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Western blot analysis of extracts of various cell lines, using MUT antibody (19-232) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using MUT antibody (19-232) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

MUT Antibody

CATALOG NUMBER: 19-232

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 451-750 of human MUT (NP_000246.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
LO2
Positive Control 2:
HeLa
Positive Control 3:
BT-474
Positive Control 4:
22Rv1
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 83kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MUT
Additional Names:
MCM, MUT, methylmalonyl-CoA mutase, mitochondrial, methylmalonyl Coenzyme A mutase, methylmalonyl-CoA isomerase, methylmalonyl-CoA mutase c.*192delA, methylmalonyl-CoA mutase c.*51C>G, methylmalonyl-CoA mutase variant c.1495G>A, methylmalonyl-CoA mutase variant c.2011A>G, methylmalonyl-CoA mutase variant c.2150G>T, methylmalonyl-CoA mutase variant c.322C>T, methylmalonyl-CoA mutase variant c.613_615delGAA, methylmalonyl-CoA mutase variant c.636G>A, methylmalonyl-CoA mutase variant c.643G>A, mutant methylmalonyl CoA mutase, truncated methylmalonyl CoA mutase, truncated methylmalonyl-CoA mutase variant c.1420C>T, truncated methylmalonyl-CoA mutase variant c.2179C>T, truncated methylmalonyl-CoA mutase variant c.91C>T
Ncbi Gene Id Number:
4594
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-232

List Size:
100 uL

List Price:

$436.00

Datasheet
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