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Western blot analysis of extracts of various cell lines, using MVP antibody (18-427) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using MVP Antibody (18-427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using MVP antibody (18-427) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MVP antibody (18-427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MVP Antibody

CATALOG NUMBER: 18-427

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MVP (NP_005106.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
HT-29
Positive Control 2:
A-549
Positive Control 3:
BT-474
Positive Control 4:
HeLa
Positive Control 5:
HepG2
Predicted Molecular Weight:
Observed: 100kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MVP
Additional Names:
MVP, Major vault protein, VAULT1, LRP
Ncbi Gene Id Number:
9961
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-427

List Size:
100 uL

List Price:

$436.00

Datasheet
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