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45-922 (xug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-922 (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-922 (xug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MYB Antibody

CATALOG NUMBER: 45-922

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QRHYNDEDPEKEKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in Human Colon lysates (calculated MW of 72.3kDa according to NP_005366.2). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Kidney shows nuclear staining in all cells. Recommended concentration: 2-4ug/ml.
Specificity:
This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq). (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1)
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MYB
Additional Names:
c-myb, MYB, v-myb myeloblastosis viral oncogene homolog (avian), c-myb_CDS, efg, Avian myeloblastosis viral (v-myb) oncogene homolog, c-myb protein (140 AA), c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog
Protein Accession Number:
NP_001123645.1,NP_005366.2,NP_001123644.1,NP_001155128.1,NP_001155129.1,NP_001155130.1,NP_001155131.1
Protein Gi Number:
46361980
Ncbi Gene Id Number:
4602
Background Reference 1:
Clappier E, Cuccuini W, Kalota A, Crinquette A, Cayuela JM, Dik WA, Langerak AW, Montpellier B, Nadel B, Walrafen P, Delattre O, Aurias A, Leblanc T, Dombret H, Gewirtz AM, Baruchel A, Sigaux F, Soulier J. The C-MYB locus is involved in chromosomal translocation and genomic duplications in human T-cell acute leukemia (T-ALL) - the translocation defining a new T-ALL subtype in very young children. Blood. 2007 Apr 23;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-922

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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