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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in mouse heart tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Myb Sumoylation Site Antibody

CATALOG NUMBER: 61-399

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Myb Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from human Myb Sumoylation Site.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYB
Additional Names:
Transcriptional activator Myb, Proto-oncogene c-Myb, MYB
Protein Accession Number:
P10242
Protein Gi Number:
2815504
Ncbi Gene Id Number:
4602
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
Background Reference 1:
Luchetti, M.M., et al., J. Biol. Chem. 278(3):1533-1541 (2003).
Background Reference 2:
Dahle, O., et al., Eur. J. Biochem. 270(6):1338-1348 (2003).
Background Reference 3:
Chen, J., et al., Oncogene 21(12):1859-1869 (2002).
Background Reference 4:
Hernandez-Munain, C., et al., J. Immunol. 169(8):4362-4369 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-399

List Size:
400 ul

List Price:

$495.00

Datasheet
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