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Western blot analysis of MYBPC1 in rat skeletal muscle tissue lysate with MYBPC1 antibody at 1 μg/mL.
Immunofluorescence of MYBPC1 in mouse skeletal muscle cells with MYBPC1 antibody at 20 μg/mL.
Western blot analysis of MYBPC1 in rat skeletal muscle tissue lysate with MYBPC1 antibody at 1 μg/mL.
Trial Size

MYBPC1 Antibody

CATALOG NUMBER: 6679

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (73%)
Immunogen:
MYBPC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human MYBPC1.
The immunogen is located within amino acids 160 - 210 of MYBPC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
MYBPC1 antibody can be used for detection of MYBPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least five isoforms of MYBPC1 are known to exist. MYBPC1 antibody is predicted not to cross-react with other MYBPC family members
Positive Control 1:
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Purification:
MYBPC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MYBPC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MYBPC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYBPC1
Additional Names:
MYBPC1 Antibody: LCCS4, MYBPCC, MYBPCS, Myosin-binding protein C, slow-type, C-protein, skeletal muscle slow isoform, Slow MyBP-C
Protein Accession Number:
NP_002456
Protein Gi Number:
45976288
Ncbi Gene Id Number:
4604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MYBPC1 Antibody: Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the slow-type isoform designated MYBPC1. Both the fast-type (MYBPC2) and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. Multiple isoforms of MYBPC1 are known to exist, and are present in varying amounts in different skeletal muscles. It is thought that the MYBPC1 slow subfamily may play important roles in the assembly and stabilization of sarcomeric M- and A-bands and regulate the contractile properties of the actomyosin filaments.
Background Reference 1:
Furst DO, Vinkemeir U and Weber K. Mammalian skeletal muscle C-protein: purification from bovine muscle, binding to titin and the characterization of a full-length human cDNA. J. Cell Sci. 1992; 102:769-78.
Background Reference 2:
Weber FE, Vaughan KT, Reiach FC, et al. Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment. Eur. J. Biochem. 1993; 216:661-9.
Background Reference 3:
Ackermann MA and Kontrogianni-Konstantopoulous A. Myosin binding protein-C slow: an intricate subfamily of proteins. J. Biomed. Biotech. 2010; 2010:652065.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6679

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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