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Western blot analysis of MYC using rabbit polyclonal MYC Antibody (S62) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MYC using rabbit polyclonal MYC Antibody (S62) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2).

MYC Antibody

CATALOG NUMBER: 60-751

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This MYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from human MYC.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYC
Additional Names:
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39
Protein Accession Number:
P01106
Protein Gi Number:
127619
Ncbi Gene Id Number:
4609
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of the gene encoding MYC have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
Background Reference 1:
Lima,F.P., Am. J. Clin. Pathol. 129 (5), 723-726 (2008)
Background Reference 2:
Ida,C., Biosci. Biotechnol. Biochem. 72 (3), 868-871 (2008)
Background Reference 3:
Iijima,S., Eur. J. Biochem. 206 (2), 595-603 (1992)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-751

List Size:
400 ul

List Price:

$495.00

Datasheet
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