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45-095 staining (0.2ug/ml) of Human Thymus lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-095 (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.
45-095 (5ug/ml) as the reporter antibody.
45-095 staining (0.2ug/ml) of Human Thymus lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

MYD88 Antibody

CATALOG NUMBER: 45-095

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-IKYKAMKKEFP
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 33kDa band observed in Human Thymus lysates (calculated MW of 33.2kDa according to NP_002459.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Recommended concentration: 4-6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MYD88 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms 1, 2 and 3 (NP_001166038.1; NP_002459.2; NP_001166039.1).
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MYD88
Additional Names:
MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88
Protein Accession Number:
NP_001166038.1,NP_002459.2,NP_001166039.1
Protein Gi Number:
4505297
Ncbi Gene Id Number:
4615
Background Reference 1:
Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med. 2005 Jan 21;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-095

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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