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45-925 (0.1ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-925 (0.1ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Myelin Protein Zero Antibody

CATALOG NUMBER: 45-925

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-DHSRSTKAVSEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Rat Spinal Cord lysates (calculated MW of 28.5kDa according to Human NP_000521.1 and of 27.7kDa according to Rat NP_058723.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
The presence of this PNS protein in Rat Spinal Cord is likely due to contamination with spinal roots during extraction from the animal.
Positive Control 1:
Rat Spinal Cord Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MPZ
Additional Names:
myelin protein zero, myelin protein zero (Charcot-Marie-Tooth neuropathy 1B) , MPZ, CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, MPP, P0
Protein Accession Number:
NP_000521.1
Protein Gi Number:
4505243
Ncbi Gene Id Number:
4359
Background Reference 1:
LeBlanc SE, Ward RM, Svaren J. Neuropathy-associated Egr2 mutants disrupt cooperative activation of myelin protein zero by Egr2 and Sox10. Mol Cell Biol. 2007 May;27(9):3521-9. Epub 2007 Feb 26.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-925

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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