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Western blot analysis of extracts of mouse skeletal muscle, using MYH6 antibody (23-876).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of mouse heart using MYH6 antibody (23-876) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using MYH6 antibody (23-876) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse skeletal muscle, using MYH6 antibody (23-876).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MYH6 Antibody

CATALOG NUMBER: 23-876

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6 (NP_002462.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 255kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYH6
Additional Names:
ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC, myosin-6, myHC-alpha, myosin heavy chain, cardiac muscle alpha isoform, myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1)
Ncbi Gene Id Number:
4624
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-876

List Size:
50 uL

List Price:

$295.00

Datasheet
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