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Western Blot at 1:1000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Western Blot at 1:1000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

MYH7 Antibody

CATALOG NUMBER: 59-342

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MYH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the N-terminal region of human MYH7.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
223 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYH7
Additional Names:
Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain, cardiac muscle beta isoform, MyHC-beta, MYH7, MYHCB
Protein Accession Number:
P12883
Protein Gi Number:
83304912
Ncbi Gene Id Number:
4625
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Muscle myosin is a hexameric protein containing 2 heavychain subunits, 2 alkali light chain subunits, and 2 regulatorylight chain subunits. This gene encodes the beta (or slow) heavychain subunit of cardiac myosin. It is expressed predominantly innormal human ventricle. It is also expressed in skeletal muscletissues rich in slow-twitch type I muscle fibers. Changes in therelative abundance of this protein and the alpha (or fast) heavysubunit of cardiac myosin correlate with the contractile velocityof cardiac muscle. Its expression is also altered during thyroidhormone depletion and hemodynamic overloading. Mutations in thisgene are associated with familial hypertrophic cardiomyopathy,myosin storage myopathy, dilated cardiomyopathy, and Laingearly-onset distal myopathy.
Background Reference 1:
Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010) :
Background Reference 2:
Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)
Background Reference 3:
Millat, G., et al. EurMed Genet 53(5):261-267(2010)
Background Reference 4:
Muelas, N., et al. Neurology 75(8):732-741(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-342

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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