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Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 5 μg/ml.
Immunofluorescence of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 20 μg/ml.
Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

MYH8 Antibody

CATALOG NUMBER: 8081

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Horse: (95%), Rabbit: (95%), Pig: (89%), Bovine: (89%), Chicken: (89%)
Immunogen:
MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8.
The immunogen is located within amino acids 60 - 110 of MYH8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 213 kDa
Observed: 220 kDa
Purification:
MYH8 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MYH8 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MYH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MYH8
Additional Names:
Myosin heavy chain 8, Myosin-8, Myosin heavy chain skeletal muscle perinatal, MyHC-perinatal, MyHC-peri, MyHC-pn, gtMHC-F
Protein Accession Number:
NP_002463
Protein Gi Number:
153945790
Ncbi Gene Id Number:
4626
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).
Background Reference 1:
Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030.
Background Reference 2:
Feghali R and Leinwand LA. Molecular genetic characterization of a developmentally regulated human perinatal myosin heavy chain. J. Cell Biol. 1989; 108:1791-7.
Background Reference 3:
Jullian EH, Kelly AM, Pompidou AJ, et al. Characterization of a human perinatal myosin heavy-chain transcript. Eur. J. Biochem. 1995; 230:1001-6.
Background Reference 4:
Minzer-Conzetti K, Wu E, Vargervik K, et al. Phenotypic variation in trismus-pseudocamptodactyly syndrome caused by a recurrent MYH8 mutation. Clin. Dysmorphol. 2008; 17:1-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8081

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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